Vista normal Vista MARC

Evaluation of horizontal gene transfer risk between the Mediterranean fruit fly Ceratitis capitata (Tephritidae) and its parasitoid Fopius ceratitivorus (Braconidae)

Ramírez Santos, Edwin Mauricio | Rendón Arana, Pedro Alfonso [autor/a] | Bourtzis, Kostas [autor/a] | Schetelig, Marc F [autor/a] | Cáceres, Carlos [autor/a] | Targovska, Asya [autor/a] | Rehling, Tanja [autor/a] | Guillén Navarro, Griselda Karina [autor/a] | Ruiz Montoya, Lorena, 1964- [autor/a] | Toledo, Jorge [autor/a] | Liedo Fernández, Pablo [autor/a].
Tipo de material: Artículo
 en línea Artículo en línea Tema(s): Ceratitis capitata | Moscas de la fruta | Parasitoides | Fopius ceratitivorus | Hymenoptera | Control biológico de plagasTema(s) en inglés: Ceratitis capitata | Fruit flies | Parasitoids | Fopius ceratitivorus | Hymenoptera | Pest control biologicalDescriptor(es) geográficos: Guatemala Nota de acceso: Acceso en línea sin restricciones En: PLoS One. volumen 13, número 12, e0207999 (December 2018), páginas 1-12. --ISSN: 1932-6203Número de sistema: 59272Resumen:
Inglés

The transgenic strain of the Mediterranean fruit fly (medfly), Ceratitis capitata (Wied.) VIENNA 8 1260, developed from the classical genetic sexing strain VIENNA 8, has two molecular markers that exhibit red fluorescence in the body and green fluorescence in testicles and sperm. These traits offer a precise tool to discriminate between mass-reared sterile males and wild fertile males, and they could potentially increase the effectiveness of control programs for this pest. To assess the risk of horizontal transfer of the fluorescence transgenes in natural ecosystems, we used the VIENNA 8 1260 strain and the medfly parasitoid Fopius ceratitivorus. The fluorescence signal and the inheritance of the fluorescence gene markers were monitored for over 16 generations (about two years) in both species using fluorescence microscopy and a PCR-based assay. The PCR analysis was performed in four independent laboratories. Both fluorescence microscopy and PCR analysis indicated that no horizontal gene transfer of the DsRed transgene occurred during 16 generations of medfly parasitoid rearing under experimental conditions.

Recurso en línea: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0207999
Etiquetas de esta biblioteca: No hay etiquetas de esta biblioteca para este título. Ingresar para agregar etiquetas.
Star ratings
    Valoración media: 0.0 (0 votos)
Existencias
Tipo de ítem Biblioteca actual Colección Signatura Estado Fecha de vencimiento Código de barras
Artículos Biblioteca Electrónica
Recursos en línea (RE)
ECOSUR Recurso digital ECO400592729064

Acceso en línea sin restricciones

The transgenic strain of the Mediterranean fruit fly (medfly), Ceratitis capitata (Wied.) VIENNA 8 1260, developed from the classical genetic sexing strain VIENNA 8, has two molecular markers that exhibit red fluorescence in the body and green fluorescence in testicles and sperm. These traits offer a precise tool to discriminate between mass-reared sterile males and wild fertile males, and they could potentially increase the effectiveness of control programs for this pest. To assess the risk of horizontal transfer of the fluorescence transgenes in natural ecosystems, we used the VIENNA 8 1260 strain and the medfly parasitoid Fopius ceratitivorus. The fluorescence signal and the inheritance of the fluorescence gene markers were monitored for over 16 generations (about two years) in both species using fluorescence microscopy and a PCR-based assay. The PCR analysis was performed in four independent laboratories. Both fluorescence microscopy and PCR analysis indicated that no horizontal gene transfer of the DsRed transgene occurred during 16 generations of medfly parasitoid rearing under experimental conditions. eng

Disponible en línea

Adobe Acrobat profesional 6.0 o superior

Con tecnología Koha