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In vitro callogenesis induction of Guarianthe skinneri (Bateman) Dressler & W.E. Higgins (Orchidaceae)

Coutiño Cortés, Ana Gabriela [autor/a] | Bertolini, Vincenzo [autor/a] | Iracheta Donjuan, Leobardo [autor/a] | Ruiz Montoya, Lorena, 1964- [autor/a] | Valle Mora, Javier Francisco [autor/a].
Tipo de material: Artículo en línea Artículo en línea Otro título: Inducción de callogénisis in vitro de Guarianthe skinneri (Bateman) Dressler & W.E. Higgins (Orchidaceae) [Título paralelo].Tema(s): Guarianthe skinneri | Orquídeas | Formación del callo | Cultivo in vitro | Sustancias de crecimiento vegetalTema(s) en inglés: Guarianthe skinneri | Orchids | Callus formation | In vitro culture | Plant growth substancesDescriptor(es) geográficos: Tapachula (Chiapas, México) Nota de acceso: Acceso en línea sin restricciones En: Acta Agronomica. volumen 66, número 2 (2017), páginas 254-259. --ISSN: 2323-0118Número de sistema: 58065Resumen:
Inglés

Guarianthe skinneri (Bateman) Dressler & W.E. Higgins., is a native orchid from Mexico, considered as threatened species by NOM-ECOL-059-SEMARNAT-2010, mainly due to the disappearance of its natural habitat and the illegal collection during its flowering season. The aim of this research was to induce in vitro callogenesis from different type of explants, using phytoregulators, in order to look for a massive production technique to contribute to its conservation. We evaluated the leaf and pseudobulb pith explants growing in semi-solid medium MS adding BAP, 2, 4-D, Kin, the interaction of BAP/2, 4-D/Kin/Sad and a control without any type of plant growth regulators. Statistical analysis showed the pseudobulb pith explants are more suitable for in vitro introduction in comparison to leaf explants, since they perform a lower percentage of contamination (18.8% in pith and 73.2% in leaves). Likewise, the pseudobulb pith explants increased callus formation (10.8%) in comparison to leaf explants (7.6%). Regarding the phytoregulators employed, BAP have allowed to increased callus formation (17%) compared to other phytoregulators (7-10%). This is the first report, which proposes the use of pseudobulb pith as explant for callus induction in G. skinneri.

Recurso en línea: http://www.revistas.unal.edu.co/index.php/acta_agronomica/article/view/57982
Lista(s) en las que aparece este ítem: Lorena Ruiz Montoya
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Acceso en línea sin restricciones

Guarianthe skinneri (Bateman) Dressler & W.E. Higgins., is a native orchid from Mexico, considered as threatened species by NOM-ECOL-059-SEMARNAT-2010, mainly due to the disappearance of its natural habitat and the illegal collection during its flowering season. The aim of this research was to induce in vitro callogenesis from different type of explants, using phytoregulators, in order to look for a massive production technique to contribute to its conservation. We evaluated the leaf and pseudobulb pith explants growing in semi-solid medium MS adding BAP, 2, 4-D, Kin, the interaction of BAP/2, 4-D/Kin/Sad and a control without any type of plant growth regulators. Statistical analysis showed the pseudobulb pith explants are more suitable for in vitro introduction in comparison to leaf explants, since they perform a lower percentage of contamination (18.8% in pith and 73.2% in leaves). Likewise, the pseudobulb pith explants increased callus formation (10.8%) in comparison to leaf explants (7.6%). Regarding the phytoregulators employed, BAP have allowed to increased callus formation (17%) compared to other phytoregulators (7-10%). This is the first report, which proposes the use of pseudobulb pith as explant for callus induction in G. skinneri. eng

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