Vista normal Vista MARC

ISSR (Inter Simple Sequence Repeats) as molecular markers to study genetic diversity in tarantulas (Araneae, Mygalomorphae)

Machkour M'Rabet, Salima [autora] | Hénaut, Yann [autor] | Dor Roques, Ariane Liliane Jeanne [autora] | Pérez Lachaud, Gabriela [autora] | Pélissier, Céline [autora] | Gers, Charles [autor/a] | Legal, Luc [autor].
Tipo de material: Artículo ArtículoTipo de contenido: Texto Tipo de medio: Computadora Tipo de portador: Recurso en líneaTema(s): Brachypelma vagans | Arañas | Estructuras genéticas | Variación genética | Inter secuencias simples repetidas | Especies en peligro de extinción | Polimorfismo (Zoología)Descriptor(es) geográficos: Ley de Fomento Agropecuario (La Misteriosa), Calakmul (Campeche, México) | Conhuas, Champotón (Campeche, México) | Zoh-Laguna (Álvaro Obregón), Calakmul (Campeche, México) | Luis Echeverría, Othón P. Blanco (Quintana Roo, México) | Raudales, Othón P. Blanco (Quintana Roo, México) | Cozumel, Cozumel (Quintana Roo, México) Nota de acceso: Disponible para usuarios de ECOSUR con su clave de acceso En: Journal of Arachnology. Volumen 37, número 1 (April 2009), páginas 10-14. --ISSN: 0161-8202Número de sistema: 22909Resumen:
Inglés

Although all species of the Brachypelma genus are protected under CITES, few studies have been performed on the genetic structure of the populations of these endangered tarantulas. Here we propose, for the first time in spiders, to use ISSR (Inter Simple Sequence Repeat) technique to study the genetic variability of Mexican populations of Brachypelma vagans (Ausserer 1875). We used a nonlethal technique to collect samples from six populations in the Yucatan peninsula and we tested seven ISSR primers. Four of these primers gave fragments (bands) that were sufficiently clear and reproducible to construct a binary matrix and determine genetic variability parameters. We revealed a very high percentage of polymorphism (P = 98.7%) the highest yet reported for tarantula spiders. Our results show that the ISSR-PCR method is promising for intraspecific variation of tarantula spiders.
Lista(s) en las que aparece este ítem: Gabriela Pérez Lachaud
Etiquetas de esta biblioteca: No hay etiquetas de esta biblioteca para este título. Ingresar para agregar etiquetas.
Star ratings
    Valoración media: 0.0 (0 votos)
Existencias
Tipo de ítem Biblioteca actual Colección Signatura Estado Fecha de vencimiento Código de barras
Artículos Biblioteca Electrónica
Recursos en línea (RE) 		ECOSUR Recurso digital ECO400229099766

Disponible para usuarios de ECOSUR con su clave de acceso

Although all species of the Brachypelma genus are protected under CITES, few studies have been performed on the genetic structure of the populations of these endangered tarantulas. Here we propose, for the first time in spiders, to use ISSR (Inter Simple Sequence Repeat) technique to study the genetic variability of Mexican populations of Brachypelma vagans (Ausserer 1875). We used a nonlethal technique to collect samples from six populations in the Yucatan peninsula and we tested seven ISSR primers. Four of these primers gave fragments (bands) that were sufficiently clear and reproducible to construct a binary matrix and determine genetic variability parameters. We revealed a very high percentage of polymorphism (P = 98.7%) the highest yet reported for tarantula spiders. Our results show that the ISSR-PCR method is promising for intraspecific variation of tarantula spiders. eng

Con tecnología Koha