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From the worm in a bottle of Mezcal: iDNA confirmation of a leech parasitizing the Antillean manatee

Pérez Flores, Jonathan Sechaly | Rueda Calderon, H [autor/a] | Kvist, S [autor/a] | Siddall, M.E [autor/a] | Oceguera Figueroa, Alejandro [autor/a].
Tipo de material: Artículo en línea Artículo en línea Tema(s): Trichechus manatus | Haementeria acuecueyetzin | Parásitos | Códigos de barras de ADNTema(s) en inglés: Trichechus manatus | Haementeria acuecueyetzin | Parasites | DNA barcodingDescriptor(es) geográficos: Palenque (Chiapas, México) Nota de acceso: Disponible para usuarios de ECOSUR con su clave de acceso En: Journal of Parasitology. volumen 102, número 5 (Oct. 2016), páginas 553-555. --ISSN: 1937-2345Número de sistema: 9625Resumen:
Inglés

Invertebrate-derived ingested DNA (iDNA) is quickly proving to be a valuable, non-invasive tool for monitoring vertebrate species of conservation concern. Using the DNA barcoding locus, we successfully identified both the blood-feeding leech Haementeria acuecueyetzin and its blood meal-the latter is shown to be derived from the Caribbean manatee, Trichechus manatus . DNA amplification was successful despite the fact that the specimen was fixed in Mezcal (a beverage distilled from agave). We report the first confirmed case of a leech feeding on a manatee, the first record of H. acuecueyetzin for the State of Chiapas and, to our knowledge, the first case of successful DNA amplification of a biological sample fixed in Mezcal other than the caterpillar "worms" more commonly found in that beverage.

Recurso en línea: http://www.bioone.org/doi/10.1645/16-46?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed
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Tipo de ítem Biblioteca actual Colección Signatura Estado Fecha de vencimiento Código de barras
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Recursos en línea (RE) 		ECOSUR Recurso digital ECO400096251443

Disponible para usuarios de ECOSUR con su clave de acceso

Invertebrate-derived ingested DNA (iDNA) is quickly proving to be a valuable, non-invasive tool for monitoring vertebrate species of conservation concern. Using the DNA barcoding locus, we successfully identified both the blood-feeding leech Haementeria acuecueyetzin and its blood meal-the latter is shown to be derived from the Caribbean manatee, Trichechus manatus . DNA amplification was successful despite the fact that the specimen was fixed in Mezcal (a beverage distilled from agave). We report the first confirmed case of a leech feeding on a manatee, the first record of H. acuecueyetzin for the State of Chiapas and, to our knowledge, the first case of successful DNA amplification of a biological sample fixed in Mezcal other than the caterpillar "worms" more commonly found in that beverage. eng

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