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Box Wilson factorial design methodology for the optimization of laccase production by Pleurotus djamor in liquid Culture

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 En: Current Research in Microbiology and Biotechnology volumen 4, número 2 (March-April 2016), páginas 835-841Nota de acceso: Acceso en línea sin restricciones Resumen:
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A surface response design methodology was used to optimize laccase production by Pleurotus djamor. Among six strains ECS-0184 was selected to apply the mathematical approach. ECS-0184 showed a specific growth rate (μ) of 0.87 days-¹ and a laccase yield (YL/X) of 372 UA g-¹ biomass. Predictable optimal conditions obtained were pH 5-6, temperature range 25-28 °C and 3-5 days of cultivation to obtain best laccase yield, 1175 UA g-¹ biomass. Critical factors for laccase production are temperature and day of harvest according with experimental design. The laccase produced by Pleurotus djamor has a molecular weight of 44 kDa; its activity was detected in PAGE gels with ABTS and pyrogallol as substrates.

Número de sistema: 8053
Lista(s) en las que aparece este ítem: José Ernesto Sánchez Vázquez
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A surface response design methodology was used to optimize laccase production by Pleurotus djamor. Among six strains ECS-0184 was selected to apply the mathematical approach. ECS-0184 showed a specific growth rate (μ) of 0.87 days-¹ and a laccase yield (YL/X) of 372 UA g-¹ biomass. Predictable optimal conditions obtained were pH 5-6, temperature range 25-28 °C and 3-5 days of cultivation to obtain best laccase yield, 1175 UA g-¹ biomass. Critical factors for laccase production are temperature and day of harvest according with experimental design. The laccase produced by Pleurotus djamor has a molecular weight of 44 kDa; its activity was detected in PAGE gels with ABTS and pyrogallol as substrates. Inglés

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