Box Wilson factorial design methodology for the optimization of laccase production by Pleurotus djamor in liquid Culture
Por: Nieto López, Consuelo [autor/a].
Márquez Rocha, Facundo Joaquín [autor/a]
| Sánchez, José E [autor/a].
Tipo de material: 
Artículo
en línea Tema(s): Pleurotus djamor | Cultivo de hongos | LacasasTema(s) en inglés: Pleurotus djamor | Fungi cultures | LacasasNota de acceso: Acceso en línea sin restricciones
En: Current Research in Microbiology and Biotechnology. volumen 4, número 2 (March-April 2016), páginas 835-841. --ISSN: 2320-2246Número de sistema: 8053Resumen:| Tipo de ítem | Biblioteca actual | Colección | Signatura | Estado | Fecha de vencimiento | Código de barras |
|---|---|---|---|---|---|---|
| Artículos | Biblioteca Electrónica Recursos en línea (RE) | ECOSUR | Recurso digital | ECO400080539166 |
Acceso en línea sin restricciones
A surface response design methodology was used to optimize laccase production by Pleurotus djamor. Among six strains ECS-0184 was selected to apply the mathematical approach. ECS-0184 showed a specific growth rate (μ) of 0.87 days-¹ and a laccase yield (YL/X) of 372 UA g-¹ biomass. Predictable optimal conditions obtained were pH 5-6, temperature range 25-28 °C and 3-5 days of cultivation to obtain best laccase yield, 1175 UA g-¹ biomass. Critical factors for laccase production are temperature and day of harvest according with experimental design. The laccase produced by Pleurotus djamor has a molecular weight of 44 kDa; its activity was detected in PAGE gels with ABTS and pyrogallol as substrates. eng
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