Pathogenicity of isolates of Serratia marcescens towards larvae of the scarab Phyllophaga blanchardi (Coleoptera)
Pineda Castellanos, Mónica L | Rodríguez Segura, Zitlhally [autor/a] | Villalobos, Francisco Javier [autor/a] | Hernández, Luciano [autor/a] | Lina, Laura [autor/a] | Nuñez Valdez, María Eugenia [autor/a].
Tipo de material:
Artículo
en línea Tema(s): Serratia marcescens
| Phyllophaga blanchardi | Escarabajos | Patogenicidad | Insecticidas biológicos | Agentes biológicos para el control de plagasTema(s) en inglés: Serratia marcescens | Phyllophaga blanchardi | Beetles | Pathogenicity | Biological insecticides | Biological pest control agentsNota de acceso: Acceso en línea sin restricciones
En: Pathogens. volumen 4, número 2 (May 2015), páginas 210-228. --ISSN: 2076-0817Número de sistema: 7011Resumen:| Tipo de ítem | Biblioteca actual | Colección | Signatura | Estado | Fecha de vencimiento | Código de barras |
|---|---|---|---|---|---|---|
| Artículos | Biblioteca Electrónica Recursos en línea (RE) | ECOSUR | Recurso digital | ECO400070119532 |
Acceso en línea sin restricciones
Serratia marcescens is a Gram negative bacterium (Enterobacteriaceae) often associated with infection of insects. In order to find pathogenic bacteria with the potential to control scarab larvae, several bacterial strains were isolated from the hemocoel of diseased Phyllophaga spp (Coleoptera:Scarabaeidae) larvae collected from cornfields in Mexico. Five isolates were identified as Serratia marcescens by 16S rRNA gene sequencing and biochemical tests. Oral and injection bioassays using healthy Phyllophaga blanchardi larvae fed with the S. marcescens isolates showed different degrees of antifeeding effect and mortality. No insecticidal activity was observed for Spodoptera frugiperda larvae (Lepidoptera: Noctuidae) by oral inoculation. S. marcescens (Sm81) cell-free culture supernatant caused significant antifeeding effect and mortality to P. blanchardi larvae by oral bioassay and also mortality by injection bioassay. Heat treated culture broths lost the ability to cause disease symptoms, suggesting the involvement of proteins in the toxic activity. A protein of 50.2 kDa was purified from the cell-free broth and showed insecticidal activity by injection bioassay towards P. blanchardi. Analysis of the insecticidal protein by tandem- mass spectrometry (LC-MS/MS) showed similarity to a Serralysin-like protein from S. marcescens spp. This insecticidal protein could have applications in agricultural biotechnology. eng
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