Methods in gut microbial ecology for ruminants / edited by Harinder P.S. Makkar, Christopher S. McSweeney

Incluye bibliografía e índice: páginas 223-225

Foreword.. Introduction.. Editorial Note.. Part 1. Designing in-vivo Microbial Ecology Studies.. 1.1. Experimental designs for rumen microbiology.. Part 2. Classical Methods for Isolation, Enumeration, Cultivation and Functional Assays of Rumen Microbes.. 2.1. Rumen bacteria.. 2.2. Bacteriophages.. 2.3. Methanogenic archaea.. 2.4. Anaerobic fungi.. 2.5. Ciliate protozoa.. Part 3. PCR-Based Methods for Analysis of Populations and Gene Expression.. 3.1. Nucleic acid extraction, oligonucleotide probes and PCR methods.. 3.2. Quantitative (real-time PCR.. Part 4. Molecular Fingerprinting Techniques for Genotypic Analysis of Pure Cultures and Microbial Communities.. 4.1. Denaturing gradient gel electrophoresis.. 4.2. Bacteriophage populations.. 4.3. Anaerobic fungal populations.. 4.4. RAPD, RFLP, T-RFLP, AFLP, RISA.. Part 5. DNA Clone Libraries of Microbial Communities.. 5.1. 16S/18S ribosomal DNA clone library analysis of rumen microbial Diversity.. Part 6. Use of Small Subunit Ribosomal RNA Directed Oligonucleotide Probes for Microbial Population Studies.. 6.1. Northern blot analysis to investigate the abundance of micro-organisms.. 6.2. Whole cell probing with fluorescently labelled probes for in situ analysis of microbial populations.. 6.3. Combined fluorescence in situ hybridization and microautoradiography (FISH-MAR.. Part 7. Genomic Analysis of Microbial Ecosystems.. 7.1. Metagenomic analysis of the microbiomes in ruminants and other herbivores.. Chapterwise Keywords.. Keyword Index

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This book presents a comprehensive up-to-date account of the methodologies and protocols for conventional and modern molecular techniques that are currently in use for studying the gut microbial ecology of ruminants. Each chapter has been contributed by experts in the field and methods have been presented in a recipe-like format designed for direct practical use in the laboratory and also to provide insight into the most appropriate techniques, their applications and the type of information that could be expected. The techniques and procedures described are also relevant and adaptable to other gastrointestinal ecosystems and the microbiology of anaerobic environments in general. This manual will demystify the methods in molecular microbial ecology for readers who are novice in the field but are excited by the prospects of the technology. It would also be invaluable for the experienced workers striving for giving new dimension to their research expanding the work in other fields and initiating cross-cutting activities. eng

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